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31.
目的 回顾性总结32例直肠癌根治会阴部造口术后二期股薄肌移植肛门成形术的治疗效果。方法 采用Williams 5级评分法对重建前后肛门功能进行评价。结果 二期股薄肌移植肛门成形术前,32例患者肛门功能均在4级以上[4级28.4%(9/32),5级71.6%(23/32)],二期股薄肌移植肛门成形术后肛门功能明显好转。结论 二期股薄肌移植肛门成形术是直肠癌根治会阴部造口术后有效的肛门重建手段。 相似文献
32.
目的初步观察用小分子干扰RNA(siRNA)沉默树突状细胞(DCs)恒定链(Ii)后,DCs疫苗的体外抗肿瘤效果。方法从小鼠骨髓分离骨髓前体细胞,细胞经100 ng/ml GM-CSF和100 ng/ml IL-4诱导培养6 d后,转染针对DCs Ii链特异的Ii-siRNA,转染后加用50 ng/ml TNF-α继续诱导细胞成熟48 h,然后分别用Western blot检测沉默效果及CCK-8试剂盒检测DCs刺激同种异体淋巴细胞增殖的能力;此外,DCs共转染Ii-siRNA和小鼠胃癌前体细胞MFC的总RNA后,与同种异体淋巴细胞共培养,通过ELISA检测培养上清IFN-γ/和IL-4的水平,并收集致敏淋巴细胞进行体外杀伤实验。结果Ii-siRNA明显抑制DCs Ii的表达。沉默Ii链能够增强DCs的淋巴细胞增殖能力,并促使淋巴细胞向Th1的方向漂移[IFN-γ:(5107±351)pg/ml,IL-4:(65±13)pg/ml,P<0.05]。淋巴细胞经共转染Ii-siRNA和MFC RNA的DCs激活后,明显而特异地杀伤靶肿瘤细胞(杀伤百分率: 66.94%±2.75%,P<0.05)。结论通过siRNA沉默DCs的Ii链可能是一种行之有效的增强抗肿瘤免疫的方法。 相似文献
33.
[目的]通过对2003~2008年自贡市食品安全投诉情况调查分析,掌握自贡市食品安全投诉的规律,为食品安全监管提供科学依据.[方法]现场调查,描述性统计分析. [结果]2003~2008年共计受理食品安全投诉66件,其中食物中毒类投诉34件(51.5%),食品感官卫生问题类投诉13件(19.7%);食品纠纷类投诉19件(28.8%).[结论]食物中毒投诉多.饮食行业的食物中毒所占比例大.家庭食物中毒造成的后果严重.加强食物中毒的监管是食品安全工作的重点.不同的食品安全投诉采用不同处理方法. 相似文献
34.
目的:介绍一种较为完善的早产儿视网膜病变(ROP)筛查模式。方法:建立基本完善的ROP登记、会诊、筛查、转诊等制度,所有工作均在新生儿重症监护病房(N ICU)完成,形成以NICU为中心的ROP筛查模式,并按此模式开展筛查工作。结果:登记符合ROP筛查标准的患儿239例,其中接受至少一次筛查的患儿222例(占应筛查总数的92.89%),按要求完成筛查的患儿176例(占应筛查总数的73.64%)。发现ROP患儿39例(占实际筛查数的17.57%),其中阈值前期1型或阈值期病变11例(占实际筛查数的4.96%)。结论:这种以NICU为中心的ROP筛查模式,制度较为完善,临床中易于操作,能有效节约医疗资源,适合在中小城市中推广。 相似文献
35.
用基因工程重组的丙肝抗原包被于硝酸纤维素膜,建立了检测丙肝IgG抗体的斑点免疫渗滤法。与ELISA试剂盒进行双盲式同步测定,二法检验结果差异无显著性。渗滤法简便快速,适用于各级医院,有很强的推广价值。 相似文献
36.
Kaufmann SJ; Sharif K; Sharma V; McVerry BA 《Human reproduction (Oxford, England)》1998,13(2):498-499
The patient was diagnosed in childhood as having severe congenital
neutropenia and had recurrent admissions with severe infections. In 1987,
prior to getting married, she was sterilized. She continued to require i.v.
antibiotics when she contracted a severe infection. On one occasion, she
was treated with growth colony stimulating factor (G- CSF). Her increased
neutrophil count was sustained following this treatment. In June 1993, she
wished to start a family and underwent in- vitro fertilization (IVF)
treatment. G-CSF was given prior to oocyte retrieval. She conceived on her
first cycle and an ultrasound scan revealed a singleton pregnancy.
Throughout the course of the pregnancy, her white cell count was monitored
closely and remained at <1.0x10(9)/l. The pregnancy progressed
uneventfully and at 37 weeks gestation she was admitted for G-CSF
injections. At 38 weeks she was delivered of a boy weighing 3350 g, by
elective Caesarean section. His white cell count was normal. This is the
first case of G-CSF being used before conception and during pregnancy in a
patient with congenital neutropenia. It shows that advances in cytokine
therapy and close interdisciplinary liaison can lead to a successful
outcome and help patients, who would otherwise remain childless, to achieve
a family.
相似文献
37.
Silber SJ; Nagy Z; Devroey P; Tournaye H; Van Steirteghem AC 《Human reproduction (Oxford, England)》1997,12(11):2422-2428
The aim of the study was to determine whether a prior diagnostic testicle
biopsy can predict success or failure of testicular sperm extraction (TESE)
with intracytoplasmic sperm injection (ICSI) in patients with
non-obstructive azoospermia caused by testicular failure, and what is the
minimum threshold of sperm production in the testis which must be surpassed
for spermatozoa to reach the ejaculate. Forty- five patients with
non-obstructive azoospermia caused by testicular failure underwent
diagnostic testicle biopsy prior to a planned future TESE-ICSI procedure.
The diagnostic testicle biopsy was analysed quantitatively, and correlated
with the quantitative findings of spermatogenesis in patients with normal
spermatogenesis, as well as with the results of subsequent attempts at
TESE-ICSI. Men with non- obstructive azoospermia caused by germinal failure
had a mean of 0-6 mature spermatids/seminiferous tubule seen on a
diagnostic testicle biopsy, compared to 17-35 mature spermatids/tubule in
men with normal spermatogenesis and obstructive azoospermia. These findings
were the same for all types of testicular failure whether Sertoli cell
only, maturation arrest, cryptorchidism, or post-chemotherapy azoospermia.
Twenty-two of 26 men with mature spermatids found in the prior testis
biopsy had successful retrieval of spermatozoa for ICSI, 12 of their
partners became pregnant, and are either ongoing or delivered. The study
suggests that 4-6 mature spermatids/tubule must be present in the testis
biopsy for any spermatozoa to reach the ejaculate. More than half of
azoospermic patients with germinal failure have minute foci of
spermatogenesis which are insufficient to produce spermatozoa in the
ejaculate. Prior diagnostic testicle biopsy analysed quantitatively (for
the presence of mature spermatids) can predict subsequent success or
failure with TESE-ICSI. Incomplete testicular failure may involve a sparse
multi-focal distribution of spermatogenesis throughout the entire testicle,
rather than a regional distribution. Therefore, it is possible that massive
testicular sampling from many different regions of the testes may not be
necessary for successful TESE-ICSI.
相似文献
38.
β-Lactoglobulin was isolated from infant formulae that were ultra high temperature (UHT) -treated, sterilized or spray-dried. The effect of the isolated β-lactoglobulin on SfaII-fimbriae-mediated adhesion of Escherichia coli to human ileostomy glycoproteins was studied in vitro. β-Lactoglobulin isolated from sterilized formulae was found to perform significantly less well than preparations from spray-dried formulae (p = 0:05). Great heterogeneity was observed in the adhesion inhibitory capacity of β-lactoglobulin isolated from UHT-treated formulae. Therefore, no significant difference was observed between UHT-treated and sterilized formulae or spray-dried formulae (p < 0:10). It can be hypothesized that β-lactoglobulin from spray-dried and some UHT-treated infant formulae may affect the colonization of mucous membranes by E. coli strains causing neonatal septicaemia and meningitis. 相似文献
39.
40.
目的 探讨阴茎折断伤的病因和诊治。方法 对2l例阴茎折断伤的临床资料进行回顾性分析。结果 致伤原因以粗暴性交13例,手淫5例,跌伤、撞击伤各l例,拒绝说明原因l例。B超确诊12例,阴茎海绵体造影确诊l例。12h内手术13例,超过12h手术3例,保守治疗5例。12h内手术组有2例发生阴茎白膜硬结,术后性功能正常;超过12h手术组和保守治疗组各有l例和2例并发阴茎硬结、阴茎成角畸形和勃起功能障碍。结论 阴茎折断致伤原因以粗暴性交和手淫为主;B超检查是诊断阴茎折断首选方法;早期手术能减少其并发症的发生;术中采用纵行小切口容易准确找到白膜破裂位置,且创伤小,适于临床应用。 相似文献